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magcellecttm rat cd3 t cell isolation kit  (R&D Systems)


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    R&D Systems magcellecttm rat cd3 t cell isolation kit
    A VACV‐neutralizing antibodies were measured in virus‐treated rats 15 and 35 days after implantation ( n = 4–5 rats per group). B Protection from subcutaneous tumor challenge after virus‐induced tumor clearance. AY‐27 cells were implanted in the flanks of cured rats ( n = 6) and naïve age‐matched control rats ( n = 4). C–E T‐cell proliferation after co‐culturing with bone marrow‐derived dendritic cells (BMDCs). CD4 + and CD8 + cells were co‐cultured with BMDCs and proliferation assayed with CellTrace Violet. The representative plots show CD4 + and CD8 + T‐cell proliferation after co‐culture with either mock‐pulsed (C) or with tumor‐lysate‐pulsed BMDCs (D). Panel (E) shows the percentage of CD4 + and CD8 + T cells that proliferated in response to BMDC stimulation ( n = 3). F, G Ex vivo upregulation of CD107a by CD8 + T cells from challenged rats. (F) <t>CD3</t> + cells were incubated +/− BMDCs for 1 h in the presence of anti‐CD107a antibody, incubated for 5 h with monensin and brefeldin A, and then stained with anti‐CD4 and anti‐CD8 antibodies. Events were gated for viable CD8 + T cells. Panel (G) shows the percentage of CD107a + CD8 + T cells +/− BMDC stimulation ( n = 3). H IFN‐γ released after 24‐h co‐culture of CD3 + cells with BMDCs ( n = 3–5). I T cells activated ex vivo by tumor‐lysate‐pulsed DCs are cytotoxic. After 6 days of co‐culture with BMDC, CD3 + cells were incubated for 18 h with 10,000 target cells and at different effector‐to‐target ratios. Lysis was determined by LDH assay. RK3E are normal rat kidney cells ( n = 2–3 performed in duplicate). Data information: Mean ± SEM is shown. Two‐way ANOVA followed by Tukey's multiple comparison test was used in (A), (B), and (H). For (A), significance was determined against the ∆ J2R group. Two‐tailed Student's t‐ test was used in (E) and (G).
    Magcellecttm Rat Cd3 T Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/magcellecttm+rat+cd3+t+cell+isolation+kit/MagCellect+Rat+B+Cell+Isolation+Kit/pmc05412795-242-10-21
    Average 93 stars, based on 34 article reviews
    magcellecttm rat cd3 t cell isolation kit - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Deletion of F4L (ribonucleotide reductase) in vaccinia virus produces a selective oncolytic virus and promotes anti‐tumor immunity with superior safety in bladder cancer models"

    Article Title: Deletion of F4L (ribonucleotide reductase) in vaccinia virus produces a selective oncolytic virus and promotes anti‐tumor immunity with superior safety in bladder cancer models

    Journal: EMBO Molecular Medicine

    doi: 10.15252/emmm.201607296

    A VACV‐neutralizing antibodies were measured in virus‐treated rats 15 and 35 days after implantation ( n = 4–5 rats per group). B Protection from subcutaneous tumor challenge after virus‐induced tumor clearance. AY‐27 cells were implanted in the flanks of cured rats ( n = 6) and naïve age‐matched control rats ( n = 4). C–E T‐cell proliferation after co‐culturing with bone marrow‐derived dendritic cells (BMDCs). CD4 + and CD8 + cells were co‐cultured with BMDCs and proliferation assayed with CellTrace Violet. The representative plots show CD4 + and CD8 + T‐cell proliferation after co‐culture with either mock‐pulsed (C) or with tumor‐lysate‐pulsed BMDCs (D). Panel (E) shows the percentage of CD4 + and CD8 + T cells that proliferated in response to BMDC stimulation ( n = 3). F, G Ex vivo upregulation of CD107a by CD8 + T cells from challenged rats. (F) CD3 + cells were incubated +/− BMDCs for 1 h in the presence of anti‐CD107a antibody, incubated for 5 h with monensin and brefeldin A, and then stained with anti‐CD4 and anti‐CD8 antibodies. Events were gated for viable CD8 + T cells. Panel (G) shows the percentage of CD107a + CD8 + T cells +/− BMDC stimulation ( n = 3). H IFN‐γ released after 24‐h co‐culture of CD3 + cells with BMDCs ( n = 3–5). I T cells activated ex vivo by tumor‐lysate‐pulsed DCs are cytotoxic. After 6 days of co‐culture with BMDC, CD3 + cells were incubated for 18 h with 10,000 target cells and at different effector‐to‐target ratios. Lysis was determined by LDH assay. RK3E are normal rat kidney cells ( n = 2–3 performed in duplicate). Data information: Mean ± SEM is shown. Two‐way ANOVA followed by Tukey's multiple comparison test was used in (A), (B), and (H). For (A), significance was determined against the ∆ J2R group. Two‐tailed Student's t‐ test was used in (E) and (G).
    Figure Legend Snippet: A VACV‐neutralizing antibodies were measured in virus‐treated rats 15 and 35 days after implantation ( n = 4–5 rats per group). B Protection from subcutaneous tumor challenge after virus‐induced tumor clearance. AY‐27 cells were implanted in the flanks of cured rats ( n = 6) and naïve age‐matched control rats ( n = 4). C–E T‐cell proliferation after co‐culturing with bone marrow‐derived dendritic cells (BMDCs). CD4 + and CD8 + cells were co‐cultured with BMDCs and proliferation assayed with CellTrace Violet. The representative plots show CD4 + and CD8 + T‐cell proliferation after co‐culture with either mock‐pulsed (C) or with tumor‐lysate‐pulsed BMDCs (D). Panel (E) shows the percentage of CD4 + and CD8 + T cells that proliferated in response to BMDC stimulation ( n = 3). F, G Ex vivo upregulation of CD107a by CD8 + T cells from challenged rats. (F) CD3 + cells were incubated +/− BMDCs for 1 h in the presence of anti‐CD107a antibody, incubated for 5 h with monensin and brefeldin A, and then stained with anti‐CD4 and anti‐CD8 antibodies. Events were gated for viable CD8 + T cells. Panel (G) shows the percentage of CD107a + CD8 + T cells +/− BMDC stimulation ( n = 3). H IFN‐γ released after 24‐h co‐culture of CD3 + cells with BMDCs ( n = 3–5). I T cells activated ex vivo by tumor‐lysate‐pulsed DCs are cytotoxic. After 6 days of co‐culture with BMDC, CD3 + cells were incubated for 18 h with 10,000 target cells and at different effector‐to‐target ratios. Lysis was determined by LDH assay. RK3E are normal rat kidney cells ( n = 2–3 performed in duplicate). Data information: Mean ± SEM is shown. Two‐way ANOVA followed by Tukey's multiple comparison test was used in (A), (B), and (H). For (A), significance was determined against the ∆ J2R group. Two‐tailed Student's t‐ test was used in (E) and (G).

    Techniques Used: Virus, Control, Derivative Assay, Cell Culture, Co-Culture Assay, Ex Vivo, Incubation, Staining, Lysis, Lactate Dehydrogenase Assay, Comparison, Two Tailed Test

    Related Articles

    Isolation:

    Article Title: Deletion of F4L (ribonucleotide reductase) in vaccinia virus produces a selective oncolytic virus and promotes anti‐tumor immunity with superior safety in bladder cancer models
    Article Snippet: .. CD3 + cells were isolated from this preparation using a MagCellectTM Rat CD3 + T‐cell isolation kit following the manufacturer's protocol (R&D Systems). ..



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    magcellecttm rat cd3 t cell isolation kit  (R&D Systems)
    93
    R&D Systems magcellecttm rat cd3 t cell isolation kit
    A VACV‐neutralizing antibodies were measured in virus‐treated rats 15 and 35 days after implantation ( n = 4–5 rats per group). B Protection from subcutaneous tumor challenge after virus‐induced tumor clearance. AY‐27 cells were implanted in the flanks of cured rats ( n = 6) and naïve age‐matched control rats ( n = 4). C–E T‐cell proliferation after co‐culturing with bone marrow‐derived dendritic cells (BMDCs). CD4 + and CD8 + cells were co‐cultured with BMDCs and proliferation assayed with CellTrace Violet. The representative plots show CD4 + and CD8 + T‐cell proliferation after co‐culture with either mock‐pulsed (C) or with tumor‐lysate‐pulsed BMDCs (D). Panel (E) shows the percentage of CD4 + and CD8 + T cells that proliferated in response to BMDC stimulation ( n = 3). F, G Ex vivo upregulation of CD107a by CD8 + T cells from challenged rats. (F) <t>CD3</t> + cells were incubated +/− BMDCs for 1 h in the presence of anti‐CD107a antibody, incubated for 5 h with monensin and brefeldin A, and then stained with anti‐CD4 and anti‐CD8 antibodies. Events were gated for viable CD8 + T cells. Panel (G) shows the percentage of CD107a + CD8 + T cells +/− BMDC stimulation ( n = 3). H IFN‐γ released after 24‐h co‐culture of CD3 + cells with BMDCs ( n = 3–5). I T cells activated ex vivo by tumor‐lysate‐pulsed DCs are cytotoxic. After 6 days of co‐culture with BMDC, CD3 + cells were incubated for 18 h with 10,000 target cells and at different effector‐to‐target ratios. Lysis was determined by LDH assay. RK3E are normal rat kidney cells ( n = 2–3 performed in duplicate). Data information: Mean ± SEM is shown. Two‐way ANOVA followed by Tukey's multiple comparison test was used in (A), (B), and (H). For (A), significance was determined against the ∆ J2R group. Two‐tailed Student's t‐ test was used in (E) and (G).
    Magcellecttm Rat Cd3 T Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/magcellecttm+rat+cd3+t+cell+isolation+kit/MagCellect+Rat+B+Cell+Isolation+Kit/pmc05412795-242-10-21
    Average 93 stars, based on 1 article reviews
    magcellecttm rat cd3 t cell isolation kit - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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    A VACV‐neutralizing antibodies were measured in virus‐treated rats 15 and 35 days after implantation ( n = 4–5 rats per group). B Protection from subcutaneous tumor challenge after virus‐induced tumor clearance. AY‐27 cells were implanted in the flanks of cured rats ( n = 6) and naïve age‐matched control rats ( n = 4). C–E T‐cell proliferation after co‐culturing with bone marrow‐derived dendritic cells (BMDCs). CD4 + and CD8 + cells were co‐cultured with BMDCs and proliferation assayed with CellTrace Violet. The representative plots show CD4 + and CD8 + T‐cell proliferation after co‐culture with either mock‐pulsed (C) or with tumor‐lysate‐pulsed BMDCs (D). Panel (E) shows the percentage of CD4 + and CD8 + T cells that proliferated in response to BMDC stimulation ( n = 3). F, G Ex vivo upregulation of CD107a by CD8 + T cells from challenged rats. (F) CD3 + cells were incubated +/− BMDCs for 1 h in the presence of anti‐CD107a antibody, incubated for 5 h with monensin and brefeldin A, and then stained with anti‐CD4 and anti‐CD8 antibodies. Events were gated for viable CD8 + T cells. Panel (G) shows the percentage of CD107a + CD8 + T cells +/− BMDC stimulation ( n = 3). H IFN‐γ released after 24‐h co‐culture of CD3 + cells with BMDCs ( n = 3–5). I T cells activated ex vivo by tumor‐lysate‐pulsed DCs are cytotoxic. After 6 days of co‐culture with BMDC, CD3 + cells were incubated for 18 h with 10,000 target cells and at different effector‐to‐target ratios. Lysis was determined by LDH assay. RK3E are normal rat kidney cells ( n = 2–3 performed in duplicate). Data information: Mean ± SEM is shown. Two‐way ANOVA followed by Tukey's multiple comparison test was used in (A), (B), and (H). For (A), significance was determined against the ∆ J2R group. Two‐tailed Student's t‐ test was used in (E) and (G).

    Journal: EMBO Molecular Medicine

    Article Title: Deletion of F4L (ribonucleotide reductase) in vaccinia virus produces a selective oncolytic virus and promotes anti‐tumor immunity with superior safety in bladder cancer models

    doi: 10.15252/emmm.201607296

    Figure Lengend Snippet: A VACV‐neutralizing antibodies were measured in virus‐treated rats 15 and 35 days after implantation ( n = 4–5 rats per group). B Protection from subcutaneous tumor challenge after virus‐induced tumor clearance. AY‐27 cells were implanted in the flanks of cured rats ( n = 6) and naïve age‐matched control rats ( n = 4). C–E T‐cell proliferation after co‐culturing with bone marrow‐derived dendritic cells (BMDCs). CD4 + and CD8 + cells were co‐cultured with BMDCs and proliferation assayed with CellTrace Violet. The representative plots show CD4 + and CD8 + T‐cell proliferation after co‐culture with either mock‐pulsed (C) or with tumor‐lysate‐pulsed BMDCs (D). Panel (E) shows the percentage of CD4 + and CD8 + T cells that proliferated in response to BMDC stimulation ( n = 3). F, G Ex vivo upregulation of CD107a by CD8 + T cells from challenged rats. (F) CD3 + cells were incubated +/− BMDCs for 1 h in the presence of anti‐CD107a antibody, incubated for 5 h with monensin and brefeldin A, and then stained with anti‐CD4 and anti‐CD8 antibodies. Events were gated for viable CD8 + T cells. Panel (G) shows the percentage of CD107a + CD8 + T cells +/− BMDC stimulation ( n = 3). H IFN‐γ released after 24‐h co‐culture of CD3 + cells with BMDCs ( n = 3–5). I T cells activated ex vivo by tumor‐lysate‐pulsed DCs are cytotoxic. After 6 days of co‐culture with BMDC, CD3 + cells were incubated for 18 h with 10,000 target cells and at different effector‐to‐target ratios. Lysis was determined by LDH assay. RK3E are normal rat kidney cells ( n = 2–3 performed in duplicate). Data information: Mean ± SEM is shown. Two‐way ANOVA followed by Tukey's multiple comparison test was used in (A), (B), and (H). For (A), significance was determined against the ∆ J2R group. Two‐tailed Student's t‐ test was used in (E) and (G).

    Article Snippet: CD3 + cells were isolated from this preparation using a MagCellectTM Rat CD3 + T‐cell isolation kit following the manufacturer's protocol (R&D Systems).

    Techniques: Virus, Control, Derivative Assay, Cell Culture, Co-Culture Assay, Ex Vivo, Incubation, Staining, Lysis, Lactate Dehydrogenase Assay, Comparison, Two Tailed Test